| 李祥栋,陆秀娟,潘虹,魏心元,曾涛,郭超,陆平,周美亮,高爱农,石明.薏苡种仁主要脂质组分分类及特征分子筛选[J].中国粮油学报,2025,40(4):41-48 |
| 薏苡种仁主要脂质组分分类及特征分子筛选 |
| Classification Of Principal Lipid Components and Distinctive Molecules Selection In Adlay Seed(Coix L.) |
| 投稿时间：2024-05-14 修订日期：2024-08-12 |
| DOI： |
| 中文关键词: 脂质组 组成差异 功能分子 薏苡 |
| 英文关键词:Lipidomics composition differention functional molecule adlay |
| 基金项目:贵州省级财政种业发展项目（黔财农[2022]45号; 黔财农[2023]2号; 黔财农[2024]14号）. |
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| 中文摘要: |
| 脂质是薏苡的重要营养和功能组分,也是薏苡天然产物开发的重要物质基础。本文以代谢组学方法剖析薏苡种仁脂类物质的分类和组成特征,为薏苡生理活性物质的挖掘利用提供科学支撑。以6份薏苡品种资源为试验材料,采用液相色谱和串联质谱(LC-MS/MS)技术对薏苡种仁的脂质组分进行全面检测。研究结果显示,6份薏苡种仁中共检测到552个脂质分子,所有脂质分子根据结构特征分为5个大类和28个亚类。甘油二酯(DG)、甘油三脂(TG)和游离脂肪酸(FAA)为薏苡脂质的主要组成亚类,三者占全部脂质含量的99.69%,以长链和不饱和脂质为主,而且栽培型薏苡的总脂质和主要脂质组分含量均优于野生资源。差异分析结果显示,共有87个脂质分子含量在不同品种类型之间存在显著差异,其中油酸(十八烯酸)、棕榈酸(十六烷酸)、二糖甘油二酯DGDG(16:0_18:2)、二糖甘油二酯DGDG(18:2_18:2)、甘油二酯DG(16:0_18:3)、单甘油酯MG(18:2)、二糖甘油二酯DGDG(18:1_18:2)、溶血磷脂酰胆碱LPC(16:0)、溶血磷脂酰胆碱LPC(18:2)9个差异分子在不同品种中含量相对较高,可作为薏苡种仁脂质组分的重要标记分子和功能组分的开发利用。 |
| 英文摘要: |
| Lipid is a category of essential nutritional and functional compositions, which was an important material basis for natural products utilization in adlay seed. In this paper, classifications and components of lipids were tested by lipidomics method that could give a scientific support for physiologically active molecule discovery and utilization in adlay. All of six coix varieties (lines) were select and then lipid components were detected comprehensively by LC-MS/MS technological method. The result was that a total of 552 lipid molecules were detected which contained 5 primary category and and 28 classes. Diglyceride (DG), triglyceride(TG) and fatty acid (FAA) were the largest amount of main classes, which account for a ratio of 99.69% in total lipid mass and dominated by long-chain and unsaturated lipids. However, all of 87 differential metabolites were found, and nine molecules of oleic acid, palmitic acid, listing as DGDG(16:0_18:2), DGDG(18:2_18:2), DG(16:0_18:3), MG(18:2), DGDG(18:1_18:2), LPC(16:0), LPC(18:2), had a higher contents in different varieties, which could act as marker molecules or use for functional component development. |
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