| 贾欣悦,徐同成,周颖,黄国清,肖军霞.pH循环法从玉米蛋白粉制备蛋白纳米颗粒的研究及其表征[J].中国粮油学报,2024,39(4):68-76 |
| pH循环法从玉米蛋白粉制备蛋白纳米颗粒的研究及其表征 |
| Study on the Fabrication of Protein Nanoparticles from Corn Gluten Meal by pH Cycling and Their Characterization |
| 投稿时间：2023-03-25 修订日期：2023-10-11 |
| DOI： |
| 中文关键词: 玉米蛋白粉 蛋白纳米颗粒 pH循环法 |
| 英文关键词:Corn gluten meal Protein nanoparticles pH cycling |
| 基金项目:山东省重点研发计划项目，国家自然科学基金项目（面上项目） |
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| 中文摘要: |
| 本文采用pH循环法直接从玉米蛋白粉（CGM）制备蛋白纳米颗粒（CNPs），对其制备工艺进行了优化，对其性质进行了表征，并与利用玉米醇溶蛋白在相同条件下制备的纳米颗粒（ZNPs）进行了比较。结果表明，利用pH循环法可以成功从CGM中制备CNPs，且其最优条件为碱溶pH 13.0、碱溶温度60℃、碱提料液比1:25、回调pH 7.0，在此条件下CNPs的得率相对于CGM中的蛋白质含量达到了69.20%。与ZNPs相比，CNPs具有相近的粒径（282.37 nm）且也携带负电荷，但是原子力显微镜观察结果表明其聚集程度更低；CNPs的疏水性氨基酸含量稍低（50.05%），但是α-螺旋和无规则卷曲的含量稍高。另外，CNPs的持水力和润湿性均显著优于ZNPs且其接触角仅为45.5°，并表现出了更好的Pickering乳液稳定能力。因此，CGM可取代玉米醇溶蛋白作为pH循环法制备蛋白纳米颗粒的原料，这一过程工艺简单、成本较低，对于丰富CGM的综合利用途径、推动玉米蛋白纳米颗粒在食品工业中的应用具有重要意义。 |
| 英文摘要: |
| The fabrication of protein nanoparticles from corn gluten meal (CGM) by pH cycling was concerned in the current work. The conditions for preparing the CGM-derived nanoparticles (CNPs) were optimized and their properties were characterized and compared with those derived from zein (ZNPs). The results indicated that protein nanoparticles could be successfully fabricated from CGM by pH cycling and the optimum conditions were extraction pH 13.0, extraction temperature 60°C, CGM to extraction solvent ratio 1:25, and cycling pH 7.0, which conferred a CNPs yield of 69.20% relative to the protein content in CGM. Compared with ZNPs, CNPs possessed similar particle size (282.37 nm) and carried negative charges as well, but was much less aggregated according to atomic force microscope observation; besides, CNPs contained less hydrophobic amino acids (50.05%) but slightly higher α-helix and random coil contents. In addition, CNPs exhibited higher water holding and wetting capabilities than ZNPs and their contact angle was as low as 45.5° with better Pickering emulsion stabilization ability. Hence, CGM is an excellent alternative source to zein for the preparation of protein nanoparticles. This work is of great significance for enriching the comprehensive utilization of CGM and promoting the application of corn protein-derived nanoparticles in the food industry. |
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