| 姚轶俊,李枝芳,王立峰,鞠兴荣.体外模拟消化对四种杂粮中酚类物质及其降脂活性的影响[J].中国粮油学报,2020,35(5):30- |
| 体外模拟消化对四种杂粮中酚类物质及其降脂活性的影响 |
| Effect of in vitro digestion on phenolic compounds and Lipid-lowering effect of four coarse cereals |
| 投稿时间：2019-08-30 修订日期：2020-02-16 |
| DOI： |
| 中文关键词: 杂粮 多酚 体外消化 HepG2细胞 降脂活性 |
| 英文关键词:coarse cereals phenolic compounds in vitro digestion HepG2 cells lipid-lowering effect |
| 基金项目:国家重点研发计划（2017YFD0401202）；江苏省农业科技自主创新项目（CX(18)3040） |
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| 中文摘要: |
| 杂粮中富含具有生理活性的多酚类化合物。本实验研究了四种常见杂粮(薏米、荞麦、青稞、红豆)模拟体外消化过程中酚类物质的变化以及其降脂活性。结果表明,经模拟体外消化后荞麦具有最高的总酚含量(27.78±0.14 mgGAE/ 100g)及黄酮增长率(57.9%)。并利用油酸诱导HepG2细胞建立高脂模型,测定以2.5mg/mL为上样浓度的四种杂粮多酚提取物干预前后细胞内TG及LDL-c含量的变化。结果表明荞麦多酚表现出了最佳的生物利用度及降脂活性,其干预组与模型组相比甘油三酯浓度下降了三分之一,达到0.156±0.032 mmol/gprot,低密度脂蛋白降至0.025±0.005 mmol/gprot,值得进一步研究其吸收与转运机制。 |
| 英文摘要: |
| Coarse cereals are rich in bioactive phenolic compounds. In this study, we evaluated changes in phytochemical contents of four kinds of coarse cereals (adlay, buckwheat, highland barley and red bean) and their lipid-lowering effect after cooking and in vitro digestion. The results showed that after in vitro digestion the total phenols in buckwheat was 27.78±0.14 mg GAE/100g, which was highest among all these coarse cereals. The total flavonoids increased after in vitro digestion in red bean, buckwheat and highland, and which increased most in buckwheat by 57.9%. Then the high-lipid model was established using HepG2 cells. And the concentration of TG and LDL-c was determined with adding of 2.5 mg/mL of polyphenols extract from four coarse cereals. The results showed that the phenolic compounds in buckwheat has good bioavailability and lipid-lowering effect (0.156±0.032 mmol/gprot of TG and 0.025±0.005 mmol/gprot of LDL-c), which merits further investigation in absorption and transport studies. |
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