| 王凤章,杨毅杰.菜籽肽的ACE、肾素抑制活性及抗氧化性研究[J].中国粮油学报,2017,32(5):50- |
| 菜籽肽的ACE、肾素抑制活性及抗氧化性研究 |
| The Research of Rapeseed Peptides'' ACE, Renin Inhibition and Antioxidant Activity |
| 投稿时间：2015-11-20 修订日期：2016-03-23 |
| DOI： |
| 中文关键词: 菜籽蛋白 膜分离 ACE抑制活性 肾素抑制活性 抗氧化性 |
| 英文关键词:Rapeseed protein membrane ultrafiltration ACE-inhibitory activity Renin antioxidant activity |
| 基金项目:国家自然科学基金项目、江苏省高校自然科学基金项目、南京市农业科技攻关计划项目 |
|
| 摘要点击次数: 2749 |
| 全文下载次数: 1017 |
| 中文摘要: |
| 本研究采用1 ku、3 ku、5 ku、10 ku的超滤膜分离菜籽蛋白嗜热菌蛋白酶水解物（TPH），得到了四种不同分子质量的菜籽肽，研究了各菜籽肽的血管紧张素转化酶-Ⅰ（ACE）抑制活性、肾素抑制活性、氧自由基吸附能力（ORAC）和细胞抗氧化（CAA）活性。结果表明：菜籽肽的分子质量与其ACE抑制活性、肾素抑制活性以及ORAC值有明显的正相关性（P<0.05）；<1 ku的菜籽肽的ORAC值远高于TPH的，其ORAC值（Trolox）高达（4363.53±45.87）μM TE/g，是谷胱甘肽（GSH）的2.4倍，其中2个菜籽肽的ORAC值与GSH相当（P<0.05）；细胞抗氧化试验表明TPH的CAA值最高，为（26.53±1.71）μmol QE/g，其次是<1 ku组分，其CAA值是（23.91±0.69）μmol QE/g。研究认为，菜籽蛋白酶水解物及各菜籽肽不仅具有ACE和肾素双重抑制活性，还有一定的氧自由基吸收能力。因此，菜籽蛋白可用于开发降血压制剂或相关功能性产品。 |
| 英文摘要: |
| In this study, rapeseed protein isolate was hydrolysed with Thermolysin to obtain rapeseed protein hydrolysates(TPHs), which were then seperated into different peptide fractions （＜1 ku, 1-3 ku, 3-5 ku, 5-10 ku）by membrane ultrafiltration. Then, antioxidant activites of TPH and different peptide fractions were evaluated by analyzing their inhibition of angiotension I-converting enzyme(ACE) and renin, oxygen radical adsorption capacity(ORAC) and celluar antioxidant activity(CAA). The results showed that there was a significant positive correlation(P<0.05) between the molecular weight of rapeseed peptides and their inhibition of ACE, renin and ORAC. The ORAC value of <1 ku group separation was (4363.53±45.87) μM TE/g, it was much higher than that of TPHs and it was 2.4 times of GSH. Two fractions were equal to that of GSH(P<0.05). Cellular antioxidant experiment showed that CAA value of TPH is the highest, with the CAA value 26.53±1.71μmol QE/g, followed by <1 ku component, the CAA value is (23.91±0.69) μmol QE/g. It was concluded that rapeseed protein hydrolysates and rapeseed peptides have high antioxidant activity, and rapeseed peptides can be used to develop natural blood pressure-lowering and other related functional foods. |
| 关闭 |
