| 罗凯,黄秀芳,胡江,王洪伟,阚建全.气相色谱质谱法检测花椒挥发油的条件优化研究[J].中国粮油学报,2013,28(8):87-91 |
| 气相色谱质谱法检测花椒挥发油的条件优化研究 |
| Study on the Optimum Condition of Gas Chromatography Mass Spectrometric Detection for Zanthoxylum Essential Oil |
| 投稿时间：2012-11-07 修订日期：2013-06-11 |
| DOI： |
| 中文关键词: 气相色谱质谱 花椒 挥发油 优化 |
| 英文关键词:Gas chromatography mass spectrometry Zanthoxylum essential Optimizion |
| 基金项目: |
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| 摘要点击次数: 15207 |
| 全文下载次数: 8 |
| 中文摘要: |
| 本文对花椒挥发油气相色谱检测的条件进行了优化。优化结果：石英毛细管柱Rtx-Wax(0.25μm×30m×0.32mm,100%聚乙二醇固定相)；程序升温,柱温40℃,保持1min,以3℃/min升至65℃,再以10℃/min升至90℃,然后以1.5℃/min升至120℃,最后以10℃/min升至230℃,保持5min；分流比25:1；载气为高纯He,柱前压为50kPa,流速为1.0mL/min；进样口温度250℃；接口温度250℃；溶剂延迟时间 2.5 min。质谱条件：EI电子源,离子源温度220℃,扫描范围35-450m/z,标准图库NIST05。方法学考察显示该条件下,其精密性、重复性和稳定性良好。该优化结果为花椒挥发油的检测和花椒品质鉴定提供了实验依据 |
| 英文摘要: |
| On zanthoxylum essential oil by gas chromatography mass spectrometry detection conditions were optimized. Result: GC conditions: Rtx-Wax capillary column, (0.25μm×30m×0.32mm, 100% PEG stationary phase); programmed temperature, column temperature 40 ℃, maintained 1 minutes, 3 ℃ / min rose to 65 ℃, then 10 ℃ / min rose to 90 ℃, and then 1.5 ℃ / min rose to 120 ℃, finally 10 ℃ / min rose to 230 ℃, maintained 5 minutes; split ratio 25:1; carrier gas was high purity He, column pressure of 50 kPa, the flow rate of 1.0mL/min; injection port temperature 250 ℃; interface temperature 250 ℃; solvent delay time of 2.5 minutes. MS conditions: EI electron source, ion source temperature of 220 ℃, scan range 35-450 m / z, the standard library NIST05. Which have good Precision, repeatability and stability. The optimization results for zanthoxylum essential Oil detection and zanthoxylum quality identification provides an experimental basis. |
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