| 孙家佳,张 智,杨可心,魏罡,宋希明.?微生物法制备松仁肽-锌螯合物及其抗氧化功能的研究[J].中国粮油学报,2020,35(5):85- |
| ?微生物法制备松仁肽-锌螯合物及其抗氧化功能的研究 |
| Study on the preparation of pine nut peptide zinc chelate by microbiological method and its antioxidant function |
| 投稿时间：2019-08-23 修订日期：2020-02-12 |
| DOI： |
| 中文关键词: 松仁肽-锌螯合物 螯合率 抗氧化 |
| 英文关键词:Pine nut peptide zinc chelate,Chelating rate, antioxidan |
| 基金项目:内蒙古自治区科技成果转化项目（OGZH2018179） |
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| 中文摘要: |
| 本文研究了微生物法制备松仁肽-锌螯合物及抗氧化性质,利用松仁粕为原料制作培养基,选用枯草芽孢杆菌(Bacillus subtilis)ls-45为发酵菌种,以硫酸锌为锌源,运用微生物法进行螯合,微生物将大分子的松仁蛋白分解为小分子肽的同时与锌离子螯合,以硫酸锌的添加量、发酵温度、发酵时间、pH 4个因素进行单因素试验和响应面试验对螯合工艺参数进行优化,以螯合率为指标,确定最佳的工艺参数为：硫酸锌添加量为0.6g,时间为50.36h,温度为38.46℃,pH值为7.41。得到最佳螯合率90.60%。通过体外抗氧化实验对松仁肽-锌螯合物、松仁肽、VC等物质的不同浓度进行DPPH 自由基清除率、还原能力、羟自由基清除率等3个指标的测定,实验结果表明松仁肽-锌螯合物的抗氧化性均高于松仁肽,并随着质量浓度的增加而呈现量效关系,由此可以初步判断松仁肽-锌螯合物具有良好的抗氧化功能。 |
| 英文摘要: |
| In this paper, we studied the preparation of pine nut peptide zinc chelate by microbiological method and its antioxidant properties. We used pine nut meal as raw material to make culture medium, Bacillus subtilis ls-45 as fermentation strain, zinc sulfate as zinc source, and microbiological method to chelate. The large molecule pine nut protein was decomposed into small molecule peptide by microbiological method and chelated with zinc ion at the same time Single factor test and response interview were carried out to optimize the chelating process parameters, including quantity, fermentation temperature, fermentation time and pH. taking chelation rate as the index, the optimal process parameters were determined as follows: The dosage of zinc sulfate is 0.6g, the time is 50.36h, the temperature is 38.46 ℃, and the pH value is 7.41. The best chelation rate was 90.60%.The DPPH free radical scavenging rate, reducing ability and hydroxyl free radical scavenging rate of pine nut peptide zinc chelate, pine nut peptide and VC in different concentrations were determined by antioxidant experiment in vitro. The experimental results showed that the antioxidant ability of pine nut peptide zinc chelate was higher than that of pine nut peptide, and showed a dose-response relationship with the increase of mass concentration, so it can be preliminarily judged that pine nut peptide -Zinc chelate has good antioxidant function. |
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