| 王强,谢跃杰,胡宝丹,任彦荣,张家蓉,王波,赵富昌,任贵礼.SPE - UHPLC - DAD法测定植物油微量酚酸类化合物的方法研究[J].中国粮油学报,2019,34(4):118-125 |
| SPE - UHPLC - DAD法测定植物油微量酚酸类化合物的方法研究 |
| Determination of nine trace phenolic acids in oil by SPE - UHPLC - DAD |
| 投稿时间：2018-06-12 修订日期：2018-07-27 |
| DOI： |
| 中文关键词: 酚酸类化合物 植物油 高效液相色谱 固相萃取 检测 |
| 英文关键词:phenolic acid compounds oil high performance liquid chromatography solid phase extraction detection |
| 基金项目: |
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| 摘要点击次数: 857 |
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| 中文摘要: |
| 建立了固相萃取-高效液相色谱对植物油中9种微量酚酸类化合物进行测定的方法。从提取及净化方式、流动相组成、流动相流速以及色谱柱温度等条件优化9种酚酸类化合物的检测方法。结果表明,样品经正己烷溶解,二醇基固相萃取柱(Diol - SPE)净化,甲醇 - 水为流动相,梯度洗脱,流速0.3 mL/min,检测波长为280 nm时,9种酚酸类化合物的检测结果最好,该检测方法的回收率在91.35 % - 103.21 %之间,日内及日间精密度的RSD范围分别在0.3 % - 0.9 %和0.6 % - 1.1 %之间。该方法快速、准确,具有较好的重复性及稳定性,适合对植物油样品中9种酚酸类化合物的检测；此外,通过对16批植物油中微量酚酸类化合物的种类及含量进行分析及对比,为植物油的分类及真假判定提供了理论基础。 |
| 英文摘要: |
| This study optimized the experimental conditions including extraction and cleanup methods, column temperature, and solvent composition, flow rate of mobile phase for nine trace phenolic acids detection by high-performance liquid chromatography (HPLC) after solid-phase extraction (SPE) cleanup. The results showed that the samples were dissolved in hexane. Diol group based solid-phase extraction column (Diol - SPE) purification, methanol and water as mobile phase, gradient elution, flow rate of 0.3 mL/min, the detection wavelength was 280 nm, detection of 9 kinds of phenolic compounds. The recovery of the detection method at the range of 91.35 % - 103.21 %. The RSD of intra-day and inter-day precision was between 0.3% - 0.9% and 0.6% -1.1%, respectively. The method was rapid, accurate, reproducible and stable. It is suitable for the determination of 9 phenolic acids in oil samples, and provides a theoretical basis for the classification and judge determination of oils by analyzing and comparing the types and contents of phenolic acids in 16 batches of oils. |
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