| 杜木香,梁珊,黄玉,庄晓慈,李爱军,汪勇.芬顿氧化降解法制备低分子量亚麻籽胶-铁复合物的研究[J].中国粮油学报,2019,34(1):81- |
| 芬顿氧化降解法制备低分子量亚麻籽胶-铁复合物的研究 |
| Fenton oxidative degradation method for preparation of low molecular weight flaxseed gum and iron complex |
| 投稿时间：2018-04-18 修订日期：2018-06-19 |
| DOI： |
| 中文关键词: 亚麻籽胶 芬顿降解 胶-铁复合物 补铁剂 理化性质 模拟消化 释放动力学 |
| 英文关键词:flaxseed gum, Fenton degradation, gum-iron complex, iron supplements, physicochemical property, simulated digestion, release kinetics |
| 基金项目:国家自然科学基金；广东省科技计划；广东省协同创新与平台环境建设专项资金；中央高校基本科研业务费专项资金项目 |
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| 中文摘要: |
| 以市售亚麻籽胶为原料，采用芬顿氧化降解法制备了低分子质量亚麻籽胶-铁复合物（LMWFG-Fe(Ⅲ)），优化了工艺条件，并进行了分离纯化、理化性质表征以及体外模拟释放动力学的研究。结果表明，在最优反应条件下（即双氧水添加量为亚麻籽胶质量的10%，Fe2+添加量为双氧水物质的量的1/2，80 ℃，pH3.0，2.0 h），亚麻籽胶平均降解率为47.83%，产品得率为43.85%，总糖含量为52.61%，多糖分子由原来的大分子质量单峰转变为4个小分子质量峰，产物的总铁含量为8.97%，其中7.86%以结合态存在。仪器分析（红外光谱，FTIR；X射线衍射，XRD）结果表明铁离子与低分子质量亚麻籽胶以共价或配位结合的方式形成了稳定的晶体网状结构。体外模拟消化动力学实验表明，LMWFG-Fe(Ⅲ)在模拟胃液中能够有效缓释铁离子，释放动力学符合Korsmeyer-Peppas模型，释放机制属非费克扩散。本研究为拓展亚麻籽胶的应用、开发新型补铁剂提供了参考。 |
| 英文摘要: |
| This study prepared a novel low molecular weight flaxseed gum and iron complex (LMWFG-Fe(Ⅲ)) by Fenton oxidative degradation method using commercial available flaxseed as raw material. The best reaction condition was screened and then separation & purification, physicochemical property characterization and in-vitro release kinetics of LMWFG-Fe(Ⅲ) were studied. Results showed that under the best reaction condition (i.e., the amount of hydrogen peroxide added was 10% of the mass of flaxseed gum; the amount of Fe2+ added was 1/2 of the molar amount of hydrogen peroxide; 80 °C; pH3.0; 2.0 h), the degradation rate of flaxseed gum was 47.83%, product yield was 43.85%, total sugar was 52.61%. Molecular weight distribution changed from a high molecular weight peak to 4 low molecular weight peaks. Total iron content in LMWFG-Fe(Ⅲ) was 8.97% and of which 7.86% existed in a bound state.. FTIR and XRD results indicate that covalent or coordinated binding between iron ions and hydroxyl or carboxyl groups in the low molecular weight flaxseed gum were presumed to happen and formed stable polysaccharide-iron crystal network structures. In-vitro release kinetics study indicates that LMWFG-Fe(Ⅲ) delayed iron release in SFG to a certain extent and the release rule trends to follow Korsmeyer-Peppas kinetics model with a non-Fickian diffusion. This study broadens the application range of flaxseed gum and provide reference for develop novel iron supplement. |
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