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李扬,章铖,邹昱成,王博,鞠兴荣.琥珀酰化改性菜籽蛋白基复合膜制备及其性能研究[J].中国粮油学报,2019,34(1):37-

琥珀酰化改性菜籽蛋白基复合膜制备及其性能研究

StudySon preparation and properties of succinylated rapeseed protein based composite films

投稿时间：2018-04-17 修订日期：2018-07-11

DOI：

英文关键词:succinylation rapeseed protein hydroxyl propyl methyl cellulose composite film

基金项目:国家自然科学基金(31501522, 31571767), 南京农业科学与技术研究项目（201505056），江苏省高校优势学科建设项目（PAPD）

作者	单位	邮编
李扬	南京财经大学	210023
章铖	南京财经大学
邹昱成	南京财经大学
王博	南京财经大学
鞠兴荣^*	南京财经大学	210023

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中文摘要:

琥珀酰化改性菜籽蛋白(SRPI)与羟丙基甲基纤维素(HPMC)复合制得薄膜。通过SDS-PAGE分析菜籽蛋白在琥珀酰化前后组分的改变,及对制备的复合膜机械性能、水蒸气透过性、透明度、二级结构和微观结构进行表征。结果表明：菜籽蛋白(RPI)经琥珀酰化改性后,30 KDa组分分子量有所增加；SRPI膜与RPI膜相比,拉伸强度提高18.7%,断裂伸长率提高38.7%,透明度提高121%,并且蛋白膜的二级结构中β-折叠与α-螺旋占比增大,无规则卷曲占比减小。此外,当SRPI:HPMC=3:1时,复合膜与SRPI膜相比阻水性提高24.1%,拉伸强度提高101.3%,α-螺旋与无规则卷曲的占比无明显变化,而β-转角占比提升,有利于蛋白膜的结构稳定,并且复合膜微观结构紧凑,均匀,这表明琥珀酰化改性菜籽蛋白与羟丙基甲基纤维素具有良好的相容性。本研究为菜籽蛋白基复合膜的开发与利用提供了理论依据。

英文摘要:

The composite films were prepared by succinylated rapeseed protein (SRPI) and hydroxypropyl methyl cellulose (HPMC). The changes of the composition of the protein molecules after succinylation were analyzed by SDS-PAGE, the mechanical properties, water vapor permeability, opacity, secondary structure and microstructure of composite films were characterized. The results showed that after succinylation, the SRPI of the molecular weight about 30 KDa increased. Compared with the RPI film, the tensile strength of SRPI film increased by 18.7%, elongation at break increased by 38.7%, opacity increased by 121%, α- helix content increased and unordered content decreased in the secondary structure. In addition, when SRPI:HPMC=3:1, the water vapor permeability of the composite film increased by 24.1%, tensile strength increased by 101.3%, α- helix content was not obviously changed and the β-turn content was promoted, which is beneficial to the stability of the protein films. The microstructure of the composite membrane is compact and uniform, which indicates the there is a good compatibility between succinylated rapeseed protein and hydroxypropyl methyl cellulose. This study provides a theoretical basis for the development and utilization of rapeseed protein based composite films..

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